PDHA1 sirna

PDHA1 siRNA (Mouse)

Cat. Num. AAA8232310

• Gene Names: Pdha1; Pdha-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDHA1; PDHA1 siRNA (Mouse); PDHA-1; Pyruvate dehydrogenase E1 component subunit alpha. somatic form mitochondrial; PDHE1-A type I; PDHA1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDHA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 390

• Applicable Applications for PDHA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDHA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDHA1 sirna

siRNA to inhibit PDHA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679261
• NCBI GeneID: 18597
• NCBI Accession #: NP_032836.1
• NCBI GenBank Nucleotide #: NM_008810.3
• UniProt Accession #: P35486
• Molecular Weight: 43,232 Da
• NCBI Official Full Name: pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
• NCBI Official Synonym Full Names: pyruvate dehydrogenase E1 alpha 1
• NCBI Official Symbol: Pdha1
• NCBI Official Synonym Symbols: Pdha-1
• NCBI Protein Information: pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
• UniProt Protein Name: Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
• Protein Family: Pyruvate dehydrogenase E1 component
• UniProt Gene Name: Pdha1
• UniProt Synonym Gene Names: Pdha-1
• UniProt Entry Name: ODPA_MOUSE

Uniprot Description

PDHA1: a mitochondrial matrix enzyme that catalyzes the oxidative decarboxylation of pyruvate, producing acetyl-CoA and CO2. A key enzyme in controlling the balance between lipid and glucose oxidation depending on substrate availability. The pyruvate dehydrogenase (PDH) holoenzyme is a multi-enzyme complex (PDHC) that contains 20-30 copies of pyruvate decarboxylase tetramers (2 alpha:2 beta)(E1), 60 copies of dihydrolipoamide acetyltransferase (E2), six homodimers of dihydrolipoamide dehydrogenase (E3), plus E3 binding proteins. The activity of PDH is tightly regulated by phosphorylation. The phosphorylation of at least one of three specific serine residues in E1 subunit by PDHK inactivates the PDHC, while dephosphorylation by PDP restores its activity. Sites 1, 2, and 3 of PDHA1 are S293, S300, and S232, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.2.4.1; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Oxidoreductase; Mitochondrial

Cellular Component: mitochondrion; intracellular membrane-bound organelle; pyruvate dehydrogenase complex; nucleus; myelin sheath

Molecular Function: pyruvate dehydrogenase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor; oxidoreductase activity; pyruvate dehydrogenase (acetyl-transferring) activity

Biological Process: acetyl-CoA biosynthetic process from pyruvate; glycolysis; tricarboxylic acid cycle; metabolic process; carbohydrate metabolic process; glucose metabolic process