OCRL sirna

OCRL siRNA (Mouse)

Cat. Num. AAA8209700

• Gene Names: Ocrl; OCRL1; BB143339; 9530014D17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OCRL; OCRL siRNA (Mouse); Inositol polyphosphate 5-phosphatase OCRL-1; OCRL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: OCRL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 900

• Applicable Applications for OCRL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OCRL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OCRL sirna

siRNA to inhibit OCRL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46195807
• NCBI GeneID: 320634
• NCBI Accession #: NP_796189.2
• NCBI GenBank Nucleotide #: NM_177215.3
• UniProt Accession #: Q6NVF0; Q8BXC9
• Molecular Weight: 84,012 Da
• NCBI Official Full Name: inositol polyphosphate 5-phosphatase OCRL-1
• NCBI Official Synonym Full Names: oculocerebrorenal syndrome of Lowe
• NCBI Official Symbol: Ocrl
• NCBI Official Synonym Symbols: OCRL1; BB143339; 9530014D17Rik
• NCBI Protein Information: inositol polyphosphate 5-phosphatase OCRL-1
• UniProt Protein Name: Inositol polyphosphate 5-phosphatase OCRL-1
• Protein Family: Inositol polyphosphate 5-phosphatase
• UniProt Gene Name: Ocrl
• UniProt Entry Name: OCRL_MOUSE

Uniprot Description

OCRL: Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5- trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5- tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes. Belongs to the inositol-1,4,5-trisphosphate 5- phosphatase type II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.3.36; Carbohydrate Metabolism - inositol phosphate

Cellular Component: Golgi apparatus; cell projection; photoreceptor outer segment; membrane; clathrin-coated vesicle; early endosome; cytoplasm; plasma membrane; coated pit; trans-Golgi network; intracellular; cytoplasmic vesicle; nucleus; endosome; cilium

Molecular Function: hydrolase activity; phosphoinositide 5-phosphatase activity; Rac GTPase binding

Biological Process: cell projection organization and biogenesis; in utero embryonic development; phosphoinositide dephosphorylation; cilium biogenesis; signal transduction