OAS1B sirna

OAS1B siRNA (Mouse)

Cat. Num. AAA8204888

• Gene Names: Oas1b; L1; Flv; Wnv; Oas1; Oias2; Mmu-L1; Oias-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: OAS1B; OAS1B siRNA (Mouse); FLV; OIAS2; Inactive 2'-5'-oligoadenylate synthase 1B; (2-5')oligo(A) synthase 1B; 2-5A synthase 1B; 2'-5'-oligoadenylate synthase-like protein 1; OAS1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: OAS1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 376

• Applicable Applications for OAS1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse OAS1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for OAS1B sirna

siRNA to inhibit OAS1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 145580613
• NCBI GeneID: 23961
• NCBI Accession #: NP_001077394.1
• NCBI GenBank Nucleotide #: NM_001083925.1
• UniProt Accession #: Q60856; Q78ZX0; Q8JZN0; Q8JZN4; Q8VI98; A8YPE5; A8YPE7; A8YPE8; A8YPE9; E9QKP6
• Molecular Weight: 43,620 Da
• NCBI Official Full Name: inactive 2'-5'-oligoadenylate synthase 1B
• NCBI Official Synonym Full Names: 2'-5' oligoadenylate synthetase 1B
• NCBI Official Symbol: Oas1b
• NCBI Official Synonym Symbols: L1; Flv; Wnv; Oas1; Oias2; Mmu-L1; Oias-2
• NCBI Protein Information: inactive 2'-5'-oligoadenylate synthase 1B
• UniProt Protein Name: Inactive 2'-5'-oligoadenylate synthase 1B
• Protein Family: Inactive 2'-5'-oligoadenylate synthase
• UniProt Gene Name: Oas1b
• UniProt Synonym Gene Names: Flv; Oias2; (2-5')oligo(A) synthase 1B; 2-5A synthase 1B
• UniProt Entry Name: OAS1B_MOUSE

NCBI Description

This gene is a member of the 2'-5' oligoA synthetase family, which are clustered on chromosome 5. The encoded protein functions in the interferon-regulated OAS/RNase L system, which mediates RNA decay as part of the innate antiviral immunity pathway. The protein binds double-stranded RNA and oligomerizes ATP, which activate the single-stranded RNA cleavage enzyme RNase L. This protein mediates resistance to flaviviruses such as West Nile virus. The majority of wild mouse strains produce a functional protein and are resistant to flavivirus infection, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a premature stop codon that inactivates this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. The full-length protein displays antiviral activity against flaviviruses such as west Nile virus (WNV) via an alternative antiviral pathway independent of RNase L. The truncated form of the protein lacks antiviral activity.