NUBPL sirna

NUBPL siRNA (Mouse)

Cat. Num. AAA8235861

• Gene Names: Nubpl; 2410170E07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NUBPL; NUBPL siRNA (Mouse); Iron-sulfur protein NUBPL; Nucleotide-binding protein-like; NUBPL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NUBPL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 319

• Applicable Applications for NUBPL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NUBPL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NUBPL sirna

siRNA to inhibit NUBPL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 167234411
• NCBI GeneID: 76826
• NCBI Accession #: NP_084036.2
• NCBI GenBank Nucleotide #: NM_029760.2
• UniProt Accession #: Q9CWD8; Q6P6K2
• Molecular Weight: 34,139 Da
• NCBI Official Full Name: iron-sulfur protein NUBPL
• NCBI Official Synonym Full Names: nucleotide binding protein-like
• NCBI Official Symbol: Nubpl
• NCBI Official Synonym Symbols: 2410170E07Rik
• NCBI Protein Information: iron-sulfur protein NUBPL
• UniProt Protein Name: Iron-sulfur protein NUBPL
• Protein Family: Iron-sulfur protein
• UniProt Gene Name: Nubpl
• UniProt Entry Name: NUBPL_MOUSE

Uniprot Description

NUBPL: Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits. Defects in NUBPL are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical manifestations from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the Mrp/NBP35 ATP-binding proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: mitochondrion

Molecular Function: metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; iron-sulfur cluster binding; ATP binding

Biological Process: mitochondrial respiratory chain complex I assembly