NSMCE1 sirna

NSMCE1 siRNA (Mouse)

Cat. Num. AAA8219641

• Gene Names: Nsmce1; 2510027N19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NSMCE1; NSMCE1 siRNA (Mouse); Non-structural maintenance of chromosomes element 1 homolog; Non-SMC element 1 homolog; NSMCE1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NSMCE1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 280

• Applicable Applications for NSMCE1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NSMCE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NSMCE1 sirna

siRNA to inhibit NSMCE1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 34328291
• NCBI GeneID: 67711
• NCBI Accession #: NP_080606.2
• NCBI GenBank Nucleotide #: NM_026330.3
• UniProt Accession #: Q9D720; Q3TLN4; Q8K2B4; Q9CY20
• Molecular Weight: 30,708 Da
• NCBI Official Full Name: non-structural maintenance of chromosomes element 1 homolog
• NCBI Official Synonym Full Names: non-SMC element 1 homolog (S. cerevisiae)
• NCBI Official Symbol: Nsmce1
• NCBI Official Synonym Symbols: 2510027N19Rik
• NCBI Protein Information: non-structural maintenance of chromosomes element 1 homolog
• UniProt Protein Name: Non-structural maintenance of chromosomes element 1 homolog
• UniProt Gene Name: Nsmce1
• UniProt Synonym Gene Names: Non-SMC element 1 homolog
• UniProt Entry Name: NSE1_MOUSE

Uniprot Description

NSMCE1: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT- associated PML bodies (APBs). Has in vitro ubiquitin ligase activity in presence of NDNL2. Is involved in positive regulation of response to DNA damage stimulus. Belongs to the NSE1 family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-

Cellular Component: nucleoplasm; chromosome, telomeric region; intracellular membrane-bound organelle; cytoplasm; chromosome; nucleus

Molecular Function: coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein ubiquitination; DNA repair; response to DNA damage stimulus; DNA recombination