NOTCH3 sirna

NOTCH3 siRNA (Mouse)

Cat. Num. AAA8238854

• Gene Names: Notch3; N3; hpbk; AW229011
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOTCH3; NOTCH3 siRNA (Mouse); Neurogenic locus notch homolog protein 3; Notch 3; NOTCH3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NOTCH3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2318

• Applicable Applications for NOTCH3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NOTCH3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOTCH3 sirna

siRNA to inhibit NOTCH3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679096
• NCBI GeneID: 18131
• NCBI Accession #: NP_032742.1
• NCBI GenBank Nucleotide #: NM_008716.2
• UniProt Accession #: Q61982
• Molecular Weight: 244,248 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: Notch3
• NCBI Official Synonym Symbols: N3; hpbk; AW229011
• NCBI Protein Information: neurogenic locus notch homolog protein 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch3
• UniProt Synonym Gene Names: Notch 3
• UniProt Entry Name: NOTC3_MOUSE

Uniprot Description

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Cellular Component: nucleoplasm; membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; nucleus; receptor complex; actin cytoskeleton

Molecular Function: protein binding; enzyme binding; calcium ion binding

Biological Process: negative regulation of neuron differentiation; negative regulation of cell differentiation; Notch signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; multicellular organismal development; forebrain development; neuron fate commitment; cell differentiation; regulation of developmental process