NLRC4 sirna

NLRC4 siRNA (Mouse)

Cat. Num. AAA8227122

• Gene Names: Nlrc4; CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NLRC4; NLRC4 siRNA (Mouse); CARD12; IPAF; NLR family CARD domain-containing protein 4; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf; NLRC4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NLRC4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,024

• Applicable Applications for NLRC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NLRC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NLRC4 sirna

siRNA to inhibit NLRC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 77736546
• NCBI GeneID: 268973
• NCBI Accession #: NP_001028539.1
• NCBI GenBank Nucleotide #: NM_001033367.3
• UniProt Accession #: Q3UP24; Q3TAU8
• Molecular Weight: 116,749 Da
• NCBI Official Full Name: NLR family CARD domain-containing protein 4
• NCBI Official Synonym Full Names: NLR family, CARD domain containing 4
• NCBI Official Symbol: Nlrc4
• NCBI Official Synonym Symbols: CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik
• NCBI Protein Information: NLR family CARD domain-containing protein 4
• UniProt Protein Name: NLR family CARD domain-containing protein 4
• Protein Family: NLR family CARD domain-containing protein
• UniProt Gene Name: Nlrc4
• UniProt Synonym Gene Names: Card12; Ipaf; Ipaf
• UniProt Entry Name: NLRC4_MOUSE

Uniprot Description

NLRC4: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: spindle microtubule; cell; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein homodimerization activity; ubiquitin-protein ligase activity; nucleotide binding; cysteine protease inhibitor activity; ATP binding

Biological Process: caspase activation; detection of bacterium; immune system process; positive regulation of apoptosis; apoptosis; regulation of signal transduction; cytokinesis; protein ubiquitination; regulation of caspase activity; activation of NF-kappaB transcription factor; regulation of apoptosis; defense response to bacterium; regulation of neuron apoptosis; innate immune response; interleukin-1 beta secretion; inflammatory response; activation of innate immune response; protein homooligomerization; negative regulation of apoptosis