MX2 sirna

MX2 siRNA (Mouse)

Cat. Num. AAA8212677

• Gene Names: Mx2; Mx-2; AI528743
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MX2; MX2 siRNA (Mouse); Interferon-induced GTP-binding protein Mx2; Myxovirus resistance protein 2; MX2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MX2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 655

• Applicable Applications for MX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MX2 sirna

siRNA to inhibit MX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7549779
• NCBI GeneID: 17858
• NCBI Accession #: NP_038634.1
• NCBI GenBank Nucleotide #: NM_013606.1
• UniProt Accession #: Q9WVP9; Q922L4
• Molecular Weight: 74,292 Da
• NCBI Official Full Name: interferon-induced GTP-binding protein Mx2
• NCBI Official Synonym Full Names: MX dynamin-like GTPase 2
• NCBI Official Symbol: Mx2
• NCBI Official Synonym Symbols: Mx-2; AI528743
• NCBI Protein Information: interferon-induced GTP-binding protein Mx2
• UniProt Protein Name: Interferon-induced GTP-binding protein Mx2
• Protein Family: Interferon-induced GTP-binding protein
• UniProt Gene Name: Mx2
• UniProt Entry Name: MX2_MOUSE

NCBI Description

This gene encodes a member of the Mx protein family of large GTPases, and functions in the innate immunity system. Interferon alpha/beta treatment or viral infection induces expression of this protein, which subsequently accumulates in the cytoplasm and inhibits viral replication. It has been shown to confer resistance to vesicular stomatitis virus, a member of the rhabdovirus family, but not to influenza virus. This gene produces a functional protein in some feral mouse strains, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a frameshift that inactivates this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

MX2: has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]

Protein type: G protein regulator, misc.

Cellular Component: cytoplasm

Molecular Function: GTPase activity; GTP binding; nucleotide binding

Biological Process: negative regulation of viral genome replication; immune system process; response to virus; innate immune response; defense response to virus