MUC1 sirna

MUC1 siRNA (Mouse)

Cat. Num. AAA8239113

• Gene Names: Muc1; EMA; CD227; Muc-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MUC1; MUC1 siRNA (Mouse); MUC-1; Mucin-1; Episialin; CD antigen CD227; MUC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MUC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 631

• Applicable Applications for MUC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MUC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MUC1 sirna

siRNA to inhibit MUC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305293
• NCBI GeneID: 17829
• NCBI Accession #: NP_038633.1
• NCBI GenBank Nucleotide #: NM_013605.2
• UniProt Accession #: Q02496
• Molecular Weight: 64,539 Da
• NCBI Official Full Name: mucin-1
• NCBI Official Synonym Full Names: mucin 1, transmembrane
• NCBI Official Symbol: Muc1
• NCBI Official Synonym Symbols: EMA; CD227; Muc-1
• NCBI Protein Information: mucin-1
• UniProt Protein Name: Mucin-1
• Protein Family: Mucin
• UniProt Gene Name: Muc1
• UniProt Synonym Gene Names: Muc-1; MUC-1; MUC1-NT; MUC1-alpha; MUC1-beta
• UniProt Entry Name: MUC1_MOUSE

Uniprot Description

MUC1: a large cell surface glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. Plays a role in adhesion and cell-cell interactions, metastasis and signaling. May provide a protective layer on epithelial surfaces. Direct or indirect interaction with actin cytoskeleton. Its cytoplasmic tail (MUC1CT) is involved in several signaling pathways, including those involving Ras, beta-catenin, p120 catenin, p53 and estrogen receptor alpha. MUC1CT also forms complexes with transcription factors, and then translocates to the nucleus by an unknown mechanism, where it is believed to influence the transcription of their target genes. MUC1CT has also been proposed to localize to mitochondrial membranes under conditions of genotoxic stress, where it attenuates the apoptotic pathway in response and confers resistance to apoptosis-inducing drugs. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Nine alternatively spliced isoforms have been described. Isoforms 5 and 9 are secreted. Isoform 7, expressed only in tumor cells, is a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling. Can bind to GRB2 adapter protein.

Protein type: Nuclear receptor co-regulator; Tumor suppressor; Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Actin-binding

Cellular Component: cell surface; membrane; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; nucleus