MTOR sirna

MTOR siRNA (Mouse)

Cat. Num. AAA827329

• Gene Names: Mtor; FRAP; flat; FRAP2; Frap1; RAFT1; RAPT1; AI327068; 2610315D21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MTOR; MTOR siRNA (Mouse); FRAP; FRAP1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin target protein; MTOR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MTOR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2549

• Applicable Applications for MTOR sirna: RNA Interference (RNAi)
• Source: Synthetic
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MTOR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Store at -20°C for one year.

Related Product Information for MTOR sirna

siRNA to inhibit MTOR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 227330586
• NCBI GeneID: 56717
• NCBI Accession #: NP_064393.2
• NCBI GenBank Nucleotide #: NM_020009.2
• UniProt Accession #: Q9JLN9; Q2KHT0; Q811J5; Q9CST1
• NCBI Official Full Name: serine/threonine-protein kinase mTOR
• NCBI Official Synonym Full Names: mechanistic target of rapamycin (serine/threonine kinase)
• NCBI Official Symbol: Mtor
• NCBI Official Synonym Symbols: FRAP; flat; FRAP2; Frap1; RAFT1; RAPT1; AI327068; 2610315D21Rik
• NCBI Protein Information: serine/threonine-protein kinase mTOR
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Mtor
• UniProt Synonym Gene Names: Frap; Frap1; mTOR; RAPT1
• UniProt Entry Name: MTOR_MOUSE

Uniprot Description

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Kinase, protein; Autophagy; EC 2.7.11.1; ATYPICAL group; PIKK family; FRAP subfamily

Cellular Component: Golgi apparatus; PML body; mitochondrion; lysosome; endoplasmic reticulum; lysosomal membrane; dendrite; endomembrane system; cytosol; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; nucleotide binding; phosphoprotein binding; ribosome binding; drug binding; transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; protein amino acid phosphorylation; response to insulin stimulus; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; cell projection organization and biogenesis; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; cell growth; regulation of response to food; regulation of GTPase activity; peptidyl-threonine phosphorylation; regulation of carbohydrate metabolic process; positive regulation of lipid biosynthetic process; negative regulation of NFAT protein import into nucleus; response to amino acid stimulus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; ruffle organization and biogenesis; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of protein kinase activity; phosphorylation