MLH1 sirna

MLH1 siRNA (Mouse)

Cat. Num. AAA8217532

• Gene Names: Mlh1; AI317206; AI325952; AI561766; 1110035C23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MLH1; MLH1 siRNA (Mouse); DNA mismatch repair protein Mlh1; MutL protein homolog 1; MLH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MLH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 760

• Applicable Applications for MLH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MLH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MLH1 sirna

siRNA to inhibit MLH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 255958238
• NCBI GeneID: 17350
• NCBI Accession #: NP_081086.2
• NCBI GenBank Nucleotide #: NM_026810.2
• UniProt Accession #: Q9JK91; Q3TG77; Q62454
• Molecular Weight: 84,670 Da
• NCBI Official Full Name: DNA mismatch repair protein Mlh1
• NCBI Official Synonym Full Names: mutL homolog 1 (E. coli)
• NCBI Official Symbol: Mlh1
• NCBI Official Synonym Symbols: AI317206; AI325952; AI561766; 1110035C23Rik
• NCBI Protein Information: DNA mismatch repair protein Mlh1
• UniProt Protein Name: DNA mismatch repair protein Mlh1
• Protein Family: Putative MutL protein
• UniProt Gene Name: Mlh1
• UniProt Entry Name: MLH1_MOUSE

Uniprot Description

MLH1: a protein involved in the repair of mismatches in DNA. Binds PMS2 or MLH1 and MLH3. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50- MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MBD4. Interacts with EXO1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2; Turcot syndrome, an autosomal dominant disorder characterized by malignant tumors of the brain; Muir-Torre syndrome (MTS), a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy; lobular carcinoma in situ; endometrial cancer; and non-polyposis colorectal cancer.

Protein type: DNA repair, damage; Tumor suppressor

Cellular Component: nucleoplasm; male germ cell nucleus; MutLalpha complex; chiasma; condensed nuclear chromosome; membrane; condensed chromosome; chromosome; synaptonemal complex; nucleus

Molecular Function: structure-specific DNA binding; ATPase activity; guanine/thymine mispair binding; MutSalpha complex binding; single-stranded DNA binding; ATP binding; mismatched DNA binding

Biological Process: oogenesis; meiosis; somatic recombination of immunoglobulin genes during immune response; double-strand break repair via nonhomologous end joining; poly(A) tail shortening; meiotic metaphase I plate congression; synapsis; male meiosis chromosome segregation; female meiosis chromosome segregation; resolution of meiotic joint molecules as recombinants; mismatch repair; somatic hypermutation of immunoglobulin genes; negative regulation of mitotic recombination; isotype switching; spindle midzone assembly involved in meiosis; DNA repair; male meiosis; cell cycle; homologous chromosome segregation; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; somatic recombination of immunoglobulin gene segments; spermatogenesis; response to DNA damage stimulus; meiotic chromosome segregation