MESP2 sirna

MESP2 siRNA (Mouse)

Cat. Num. AAA8225490

• Gene Names: Mesp2; bHLHc6
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MESP2; MESP2 siRNA (Mouse); Mesoderm posterior protein 2; MESP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MESP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for MESP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MESP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MESP2 sirna

siRNA to inhibit MESP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 111955348
• NCBI GeneID: 17293
• NCBI Accession #: NP_032615.2
• NCBI GenBank Nucleotide #: NM_008589.2
• UniProt Accession #: O08574; Q3UIV5
• Molecular Weight: 39,789 Da
• NCBI Official Full Name: mesoderm posterior protein 2
• NCBI Official Synonym Full Names: mesoderm posterior 2
• NCBI Official Symbol: Mesp2
• NCBI Official Synonym Symbols: bHLHc6
• NCBI Protein Information: mesoderm posterior protein 2
• UniProt Protein Name: Mesoderm posterior protein 2
• Protein Family: Mesoderm posterior protein
• UniProt Gene Name: Mesp2
• UniProt Entry Name: MESP2_MOUSE

Uniprot Description

MESP2: Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defects in MESP2 are the cause of spondylocostal dysostosis type 2 (SCDO2). An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life.

Protein type: Cell development/differentiation; Transcription factor

Cellular Component: nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; somitogenesis; Notch signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; mesodermal cell migration; somite rostral/caudal axis specification