MAML1 sirna

MAML1 siRNA (Mouse)

Cat. Num. AAA828320

• Gene Names: Maml1; Mam1; Mam-1; AI644666; AW743257; mKIAA0200; D930008C07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAML1; MAML1 siRNA (Mouse); KIAA0200; Mastermind-like protein 1; Mam-1; MAML1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAML1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1020

• Applicable Applications for MAML1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAML1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAML1 sirna

siRNA to inhibit MAML1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157838023
• NCBI GeneID: 103806
• NCBI Accession #: NP_780543.2
• NCBI GenBank Nucleotide #: NM_175334.3
• UniProt Accession #: Q6T264; Q505D8; Q5SUC2; Q6PDK3; Q6ZQG5; Q8BIU5; Q8R3T0
• Molecular Weight: 107,726 Da
• NCBI Official Full Name: mastermind-like protein 1
• NCBI Official Synonym Full Names: mastermind like 1 (Drosophila)
• NCBI Official Symbol: Maml1
• NCBI Official Synonym Symbols: Mam1; Mam-1; AI644666; AW743257; mKIAA0200; D930008C07Rik
• NCBI Protein Information: mastermind-like protein 1
• UniProt Protein Name: Mastermind-like protein 1
• Protein Family: Mastermind-like protein
• UniProt Gene Name: Maml1
• UniProt Synonym Gene Names: Mam-1
• UniProt Entry Name: MAML1_MOUSE

Uniprot Description

MAML1: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. Belongs to the mastermind family.

Protein type: Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear speck; nucleus

Molecular Function: peptide antigen binding; transcription coactivator activity; protein kinase binding

Biological Process: Notch signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; activation of Notch receptor target transcription factor