MAEA sirna

MAEA siRNA (Mouse)

Cat. Num. AAA8235326

• Gene Names: Maea; EMP; Gid9; 1110030D19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAEA; MAEA siRNA (Mouse); EMP; Macrophage erythroblast attacher; Erythroblast macrophage protein; MAEA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MAEA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 396

• Applicable Applications for MAEA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MAEA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAEA sirna

siRNA to inhibit MAEA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 120407043
• NCBI GeneID: 59003
• NCBI Accession #: NP_067475.2
• NCBI GenBank Nucleotide #: NM_021500.2
• UniProt Accession #: Q4VC33; Q3TFH0; Q3U7T6; Q5XKE6; Q8BPI3; Q8BSA1; Q9JK49
• Molecular Weight: 50,602 Da
• NCBI Official Full Name: macrophage erythroblast attacher
• NCBI Official Synonym Full Names: macrophage erythroblast attacher
• NCBI Official Symbol: Maea
• NCBI Official Synonym Symbols: EMP; Gid9; 1110030D19Rik
• NCBI Protein Information: macrophage erythroblast attacher
• UniProt Protein Name: Macrophage erythroblast attacher
• Protein Family: Macrophage erythroblast attacher
• UniProt Gene Name: Maea
• UniProt Synonym Gene Names: Emp
• UniProt Entry Name: MAEA_MOUSE

Uniprot Description

MAEA: Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA- mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Cell adhesion; Actin-binding; Membrane protein, integral

Cellular Component: nucleoplasm; cytoskeleton; nuclear matrix; membrane; integral to plasma membrane; cytoplasm; plasma membrane; contractile ring; spindle; nucleus; actin cytoskeleton

Molecular Function: actin binding

Biological Process: negative regulation of myeloid cell apoptosis; erythrocyte development; erythrocyte maturation; cell division; enucleate erythrocyte development; cytoskeleton organization and biogenesis; cell adhesion; cell cycle