MACROD1 sirna

MACROD1 siRNA (Mouse)

Cat. Num. AAA8230593

• Gene Names: Macrod1; Lrp16; AI604841; AW743046; D930010J01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MACROD1; MACROD1 siRNA (Mouse); LRP16; O-acetyl-ADP-ribose deacetylase MACROD1; MACRO domain-containing protein 1; Protein LRP16; [Protein ADP-ribosylglutamate] hydrolase; MACROD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MACROD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for MACROD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MACROD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MACROD1 sirna

siRNA to inhibit MACROD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 170650601
• NCBI GeneID: 107227
• NCBI Accession #: NP_598908.2
• NCBI GenBank Nucleotide #: NM_134147.4
• UniProt Accession #: Q922B1
• Molecular Weight: 35,295 Da
• NCBI Official Full Name: O-acetyl-ADP-ribose deacetylase MACROD1
• NCBI Official Synonym Full Names: MACRO domain containing 1
• NCBI Official Symbol: Macrod1
• NCBI Official Synonym Symbols: Lrp16; AI604841; AW743046; D930010J01Rik
• NCBI Protein Information: O-acetyl-ADP-ribose deacetylase MACROD1
• UniProt Protein Name: O-acetyl-ADP-ribose deacetylase MACROD1
• Protein Family: O-acetyl-ADP-ribose deacetylase
• UniProt Gene Name: Macrod1
• UniProt Synonym Gene Names: Lrp16
• UniProt Entry Name: MACD1_MOUSE

Uniprot Description

MACROD1: Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity. A chromosomal aberration involving MACROD1 is found in acute leukemia. Translocation t(11;21)(q13;q22) that forms a RUNX1-MACROD1 fusion protein.

Protein type: EC 3.5.1.-; Mitochondrial

Cellular Component: mitochondrion; nucleus

Molecular Function: (3,5-dichlorophenylurea)acetate amidohydrolase activity; single-strand selective uracil DNA N-glycosylase activity; deacetylase activity; uracil DNA N-glycosylase activity; hydrolase activity; DNA N-glycosylase activity; hydrolase activity, acting on glycosyl bonds; purine-specific mismatch base pair DNA N-glycosylase activity; UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity; N-acetylgalactosamine-6-phosphate deacetylase activity; N2-acetyl-L-lysine deacetylase activity; DNA-3-methyladenine glycosylase III activity; iprodione amidohydrolase activity; indoleacetamide hydrolase activity; protein N-terminal asparagine amidohydrolase activity; histone deacetylase activity; O-succinylbenzoate synthase activity

Biological Process: protein amino acid de-ADP-ribosylation; purine nucleoside metabolic process; response to DNA damage stimulus