LRAT sirna

LRAT siRNA (Mouse)

Cat. Num. AAA8215231

• Gene Names: Lrat; AI449251; 1300010A18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LRAT; LRAT siRNA (Mouse); Lecithin retinol acyltransferase; Phosphatidylcholine--retinol O-acyltransferase; Phosphatidylcholine-retinol-O-acyltransferase; LRAT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LRAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 231

• Applicable Applications for LRAT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LRAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LRAT sirna

siRNA to inhibit LRAT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12963753
• NCBI GeneID: 79235
• NCBI Accession #: NP_076113.1
• NCBI GenBank Nucleotide #: NM_023624.4
• UniProt Accession #: Q9JI60
• Molecular Weight: 25,820 Da
• NCBI Official Full Name: lecithin retinol acyltransferase
• NCBI Official Synonym Full Names: lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase)
• NCBI Official Symbol: Lrat
• NCBI Official Synonym Symbols: AI449251; 1300010A18Rik
• NCBI Protein Information: lecithin retinol acyltransferase
• UniProt Protein Name: Lecithin retinol acyltransferase
• Protein Family: Lecithin retinol acyltransferase
• UniProt Gene Name: Lrat
• UniProt Entry Name: LRAT_MOUSE

Uniprot Description

LRAT: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14). It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the H-rev107 family.

Protein type: Transferase; Membrane protein, integral; EC 2.3.1.135; Cofactor and Vitamin Metabolism - retinol

Cellular Component: multivesicular body; rough endoplasmic reticulum; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; integral to membrane; endosome

Molecular Function: transferase activity; retinoic acid binding; transferase activity, transferring acyl groups; O-acyltransferase activity; retinol binding; phosphatidylcholine-retinol O-acyltransferase activity

Biological Process: positive regulation of lipid transport; embryonic development; visual perception; retinol metabolic process; response to stimulus; lecithin metabolic process; vitamin A metabolic process