LDHB sirna

LDHB siRNA (Mouse)

Cat. Num. AAA8232788

• Gene Names: Ldhb; Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LDHB; LDHB siRNA (Mouse); LDH-2; LDH2; L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; LDHB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LDHB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 259

• Applicable Applications for LDHB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LDHB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LDHB sirna

siRNA to inhibit LDHB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 718551069
• NCBI GeneID: 16832
• NCBI Accession #: NP_001289694.1
• NCBI GenBank Nucleotide #: NM_001302765.1
• UniProt Accession #: P16125; Q545Y4
• Molecular Weight: 36,572 Da
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform 2
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: Ldhb
• NCBI Official Synonym Symbols: Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldhb
• UniProt Synonym Gene Names: Ldh-2; Ldh2; LDH-B; LDH-H
• UniProt Entry Name: LDHB_MOUSE

NCBI Description

This gene encodes a subunit of the lactate dehydrogenase enzyme, which catalyzes the reversible conversion of lactate to pyruvate in the glycolytic pathway. This protein is one of two lactate dehydrogenase subunits that combine to form five distinct isozymes with different kinetic parameters. Pseudogenes have been identified on chromosomes 1 and 19. Alternative splicing of this gene results in multiple transcript variants that encode multiple isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

LDH-B: an enzyme that participates in the final step of anaerobic glycolysis. Catalyzes the conversion of (S)-lactate + NAD+ into pyruvate + NADH.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; EC 1.1.1.27; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - cysteine and methionine

Cellular Component: mitochondrion; membrane; cytoplasm; myelin sheath

Molecular Function: identical protein binding; lactate dehydrogenase activity; oxidoreductase activity; NAD binding; catalytic activity; kinase binding; L-lactate dehydrogenase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: NAD metabolic process; carbohydrate metabolic process; lactate metabolic process; carboxylic acid metabolic process; lactate biosynthetic process from pyruvate