KIF3A sirna

KIF3A siRNA (Mouse)

Cat. Num. AAA8221881

• Gene Names: Kif3a; Kif3; Kifl; Kns3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KIF3A; KIF3A siRNA (Mouse); KIF3; Kinesin-like protein KIF3A; Microtubule plus end-directed kinesin motor 3A; KIF3A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KIF3A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 698

• Applicable Applications for KIF3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KIF3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KIF3A sirna

siRNA to inhibit KIF3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 595763312
• NCBI GeneID: 16568
• NCBI Accession #: NP_001277734.1
• NCBI GenBank Nucleotide #: NM_001290805.1
• UniProt Accession #: P28741; Q7TSZ7
• Molecular Weight: 80,170 Da
• NCBI Official Full Name: kinesin-like protein KIF3A isoform 2
• NCBI Official Synonym Full Names: kinesin family member 3A
• NCBI Official Symbol: Kif3a
• NCBI Official Synonym Symbols: Kif3; Kifl; Kns3
• NCBI Protein Information: kinesin-like protein KIF3A
• UniProt Protein Name: Kinesin-like protein KIF3A
• Protein Family: Kinesin-like protein
• UniProt Gene Name: Kif3a
• UniProt Synonym Gene Names: Kif3
• UniProt Entry Name: KIF3A_MOUSE

Uniprot Description

KIF3A: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Heterodimer of KIF3A and KIF3B. Interacts with PIFO/C1orf88. Interacts with CLN3. Belongs to the kinesin-like protein family. Kinesin II subfamily.

Protein type: Motor; Microtubule-binding

Cellular Component: microtubule cytoskeleton; kinesin complex; microtubule; centrosome; synaptic vesicle; neuron projection; cell projection; cytoskeleton; spindle microtubule; cytoplasm; kinesin II complex; axoneme; photoreceptor connecting cilium; cilium

Molecular Function: protein binding; spectrin binding; microtubule binding; ATPase activity; protein complex binding; nucleotide binding; microtubule motor activity; ATP binding

Biological Process: smoothened signaling pathway; epidermis development; in utero embryonic development; heart development; microtubule-based movement; positive regulation of receptor-mediated endocytosis; anterior/posterior pattern formation; inner ear receptor stereocilium organization and biogenesis; cell projection organization and biogenesis; dorsal/ventral pattern formation; cilium biogenesis; kidney development; determination of left/right symmetry; dorsoventral neural tube patterning; positive regulation of epithelial cell proliferation