KDM2B sirna

KDM2B siRNA (Mouse)

Cat. Num. AAA8215542

• Gene Names: Kdm2b; Cxxc2; Fbl10; PCCX2; Fbxl10; Jhdm1b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KDM2B; KDM2B siRNA (Mouse); FBL10; FBXL10; JHDM1B; KIAA3014; Lysine-specific demethylase 2B; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; JmjC domain-containing histone demethylation protein 1B; [Histone-H3]-lysine-36 demethylase 1B; KDM2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KDM2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1309

• Applicable Applications for KDM2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KDM2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KDM2B sirna

siRNA to inhibit KDM2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 54607022
• NCBI GeneID: 30841
• NCBI Accession #: NP_001003953.1
• NCBI GenBank Nucleotide #: NM_001003953.2
• UniProt Accession #: Q6P1G2; Q3V396; Q6PFD0; Q6ZPE8; Q9CSF7; Q9QZN6
• Molecular Weight: 12,956 Da
• NCBI Official Full Name: lysine-specific demethylase 2B isoform 1
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 2B
• NCBI Official Symbol: Kdm2b
• NCBI Official Synonym Symbols: Cxxc2; Fbl10; PCCX2; Fbxl10; Jhdm1b
• NCBI Protein Information: lysine-specific demethylase 2B
• UniProt Protein Name: Lysine-specific demethylase 2B
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: Kdm2b
• UniProt Synonym Gene Names: Fbl10; Fbxl10; Jhdm1b; Kiaa3014
• UniProt Entry Name: KDM2B_MOUSE

NCBI Description

The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

FBXL10: Histone demethylase that demethylates 'Lys-4' and 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Belongs to the JHDM1 histone demethylase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; Nucleolus; EC 1.14.11.27; Oxidoreductase; DNA-binding

Cellular Component: PcG protein complex; nucleus

Molecular Function: rRNA binding; protein binding; histone demethylase activity; DNA binding; dioxygenase activity; zinc ion binding; RNA binding; histone demethylase activity (H3-K36 specific); metal ion binding; oxidoreductase activity

Biological Process: midbrain-hindbrain boundary morphogenesis; transcription, DNA-dependent; fourth ventricle development; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; third ventricle development; initiation of neural tube closure; histone demethylation; regulation of transcription, DNA-dependent; forebrain development; midbrain development; spermatogenesis; negative regulation of neuron apoptosis; embryonic camera-type eye morphogenesis; lateral ventricle development; hindbrain development