KCNJ3 sirna
KCNJ3 siRNA (Mouse)
Gene Names
Kcnj3; GIRK1; Kcnf3; GIRK-1; Kir3.1
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
KCNJ3; KCNJ3 siRNA (Mouse); GIRK1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K(+) channel Kir3.1; Potassium channel. inwardly rectifying subfamily J member 3; KCNJ3 sirna
Host
Synthetic
Reactivity
Mouse
Specificity
KCNJ3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
501
Applicable Applications for KCNJ3 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KCNJ3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for KCNJ3 sirna
siRNA to inhibit KCNJ3 expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,573 Da
NCBI Official Full Name
G protein-activated inward rectifier potassium channel 1 isoform 1
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 3
NCBI Official Symbol
Kcnj3
NCBI Official Synonym Symbols
GIRK1; Kcnf3; GIRK-1; Kir3.1
NCBI Protein Information
G protein-activated inward rectifier potassium channel 1
UniProt Protein Name
G protein-activated inward rectifier potassium channel 1
Protein Family
UniProt Gene Name
Kcnj3
UniProt Synonym Gene Names
Girk1; GIRK-1
UniProt Entry Name
KCNJ3_MOUSE
Uniprot Description
GIRK1: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.
Protein type: Membrane protein, integral; Channel, potassium; Membrane protein, multi-pass
Cellular Component: cell surface; membrane; integral to plasma membrane; T-tubule; integral to membrane; external side of plasma membrane
Molecular Function: G-protein activated inward rectifier potassium channel activity; voltage-gated ion channel activity; inward rectifier potassium channel activity
Biological Process: potassium ion import; transport; ion transport; potassium ion transport
