ITPR2 sirna

ITPR2 siRNA (Mouse)

Cat. Num. AAA8201449

• Gene Names: Itpr2; Ip3r2; Itpr5; insP3R2; AI649341; InsP3R-2; InsP3R-5
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITPR2; ITPR2 siRNA (Mouse); ITPR5; Inositol 1.4.5-trisphosphate receptor type 2; IP3 receptor isoform 2; IP3R 2; InsP3R2; Inositol 1.4.5-trisphosphate type V receptor; Type 2 inositol 1.4.5-trisphosphate receptor; Type 2 InsP3 receptor; ITPR2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ITPR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2668

• Applicable Applications for ITPR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ITPR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITPR2 sirna

siRNA to inhibit ITPR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 60592758
• NCBI GeneID: 16439
• NCBI Accession #: NP_034716.1
• NCBI GenBank Nucleotide #: NM_010586.2
• UniProt Accession #: Q9Z329; P70226; Q5DWM3; Q5DWM5; Q61744; Q8R3B0; B2KF91
• Molecular Weight: 304,036 Da
• NCBI Official Full Name: inositol 1,4,5-trisphosphate receptor type 2 isoform 2
• NCBI Official Synonym Full Names: inositol 1,4,5-triphosphate receptor 2
• NCBI Official Symbol: Itpr2
• NCBI Official Synonym Symbols: Ip3r2; Itpr5; insP3R2; AI649341; InsP3R-2; InsP3R-5
• NCBI Protein Information: inositol 1,4,5-trisphosphate receptor type 2
• UniProt Protein Name: Inositol 1,4,5-trisphosphate receptor type 2
• Protein Family: Inositol 1,4,5-trisphosphate receptor
• UniProt Gene Name: Itpr2
• UniProt Synonym Gene Names: Itpr5; IP3R 2; InsP3R2; Type 2 InsP3 receptor
• UniProt Entry Name: ITPR2_MOUSE

Uniprot Description

IP3R2: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA. Most normal tissues displayed weak to moderate cytoplasmic positivity. Strong staining was observed in the gastrointestinal tract, hepatocytes and exocrine pancreas. Short isoform Is found in skeletal muscle and heart. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylation by PKA increases calcium release. Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor. Belongs to the InsP3 receptor family. 2 isoforms of the human protein are produced by alternative splicing. The short isoform is found in skeletal muscle and heart.

Protein type: Channel, calcium; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; sarcoplasmic reticulum; membrane; endoplasmic reticulum; axon; cytoplasm; integral to membrane; plasma membrane; cell cortex; myelin sheath; receptor complex

Molecular Function: calcium-release channel activity; calcium channel activity; ion channel activity; calcium ion binding; phosphoinositide binding; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity

Biological Process: inositol phosphate-mediated signaling; transport; calcium ion transport; response to hypoxia; ion transport; transmembrane transport