ID4 sirna

ID4 siRNA (Mouse)

Cat. Num. AAA8231180

• Gene Names: Id4; Idb4; bHLHb27
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ID4; ID4 siRNA (Mouse); ID-4; IDB4; DNA-binding protein inhibitor ID-4; Inhibitor of DNA binding 4; ID4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ID4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for ID4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ID4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ID4 sirna

siRNA to inhibit ID4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13624309
• NCBI GeneID: 15904
• NCBI Accession #: NP_112443.1
• NCBI GenBank Nucleotide #: NM_031166.2
• UniProt Accession #: P41139
• Molecular Weight: 16,596 Da
• NCBI Official Full Name: DNA-binding protein inhibitor ID-4
• NCBI Official Synonym Full Names: inhibitor of DNA binding 4
• NCBI Official Symbol: Id4
• NCBI Official Synonym Symbols: Idb4; bHLHb27
• NCBI Protein Information: DNA-binding protein inhibitor ID-4
• UniProt Protein Name: DNA-binding protein inhibitor ID-4
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: Id4
• UniProt Synonym Gene Names: Id-4; Idb4
• UniProt Entry Name: ID4_MOUSE

Uniprot Description

ID4: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.

Protein type: Transcription, coactivator/corepressor

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding

Biological Process: fat cell differentiation; transcription, DNA-dependent; myelination in the central nervous system; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; cerebral cortex neuron differentiation; negative regulation of oligodendrocyte differentiation; cell proliferation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; regulation of transcription, DNA-dependent; negative regulation of astrocyte differentiation; neuroblast proliferation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle