ICMT sirna

ICMT siRNA (Mouse)

Cat. Num. AAA8240576

• Gene Names: Icmt; PPMT; STE14; pcCMT; C80758; HSTE14; Gm13095; 1700008E11Rik; OTTMUSG00000010406
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ICMT; ICMT siRNA (Mouse); Protein-S-isoprenylcysteine O-methyltransferase; Isoprenylcysteine carboxylmethyltransferase; Prenylated protein carboxyl methyltransferase; PPMT; Prenylcysteine carboxyl methyltransferase; pcCMT; ICMT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ICMT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 284

• Applicable Applications for ICMT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ICMT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ICMT sirna

siRNA to inhibit ICMT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 51317397
• NCBI GeneID: 57295
• NCBI Accession #: NP_598549.1
• NCBI GenBank Nucleotide #: NM_133788.2
• UniProt Accession #: Q9EQK7
• Molecular Weight: 31,790 Da
• NCBI Official Full Name: protein-S-isoprenylcysteine O-methyltransferase
• NCBI Official Synonym Full Names: isoprenylcysteine carboxyl methyltransferase
• NCBI Official Symbol: Icmt
• NCBI Official Synonym Symbols: PPMT; STE14; pcCMT; C80758; HSTE14; Gm13095; 1700008E11Rik; OTTMUSG00000010406
• NCBI Protein Information: protein-S-isoprenylcysteine O-methyltransferase
• UniProt Protein Name: Protein-S-isoprenylcysteine O-methyltransferase
• Protein Family: Protein-S-isoprenylcysteine O-methyltransferase
• UniProt Gene Name: Icmt
• UniProt Synonym Gene Names: PPMT; pcCMT
• UniProt Entry Name: ICMT_MOUSE

Uniprot Description

ICMT: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Belongs to the isoprenylcysteine O-methyltransferase family.

Protein type: EC 2.1.1.100; Methyltransferase; Endoplasmic reticulum; Membrane protein, multi-pass; Transcription, coactivator/corepressor; Membrane protein, integral

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function: transferase activity; methyltransferase activity; protein-S-isoprenylcysteine O-methyltransferase activity; cAMP response element binding protein binding

Biological Process: methylation; regulation of Ras protein signal transduction; protein localization; protein amino acid methylation; in utero embryonic development; C-terminal protein amino acid methylation; multicellular organism growth; positive regulation of cell proliferation; S-adenosylmethioninamine metabolic process; S-adenosylhomocysteine metabolic process; liver development