HSP90AB1 sirna

HSP90AB1 siRNA (Mouse)

Cat. Num. AAA8208540

• Gene Names: Hsp90ab1; 90kDa; Hsp84; Hsp90; Hspcb; C81438; Hsp84-1; AL022974
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSP90AB1; HSP90AB1 siRNA (Mouse); HSP84; HSP84-1; HSPCB; Heat shock protein HSP 90-beta; Heat shock 84 kDa; HSP 84; Tumor-specific transplantation 84 kDa antigen; TSTA; HSP90AB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HSP90AB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 724

• Applicable Applications for HSP90AB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HSP90AB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSP90AB1 sirna

siRNA to inhibit HSP90AB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40556608
• NCBI GeneID: 15516
• NCBI Accession #: NP_032328.2
• NCBI GenBank Nucleotide #: NM_008302.3
• UniProt Accession #: P11499; O89078; Q3UIQ7
• Molecular Weight: 83,281 Da
• NCBI Official Full Name: heat shock protein HSP 90-beta
• NCBI Official Synonym Full Names: heat shock protein 90 alpha (cytosolic), class B member 1
• NCBI Official Symbol: Hsp90ab1
• NCBI Official Synonym Symbols: 90kDa; Hsp84; Hsp90; Hspcb; C81438; Hsp84-1; AL022974
• NCBI Protein Information: heat shock protein HSP 90-beta
• UniProt Protein Name: Heat shock protein HSP 90-beta
• Protein Family: Heat shock protein
• UniProt Gene Name: Hsp90ab1
• UniProt Synonym Gene Names: Hsp84; Hsp84-1; Hspcb; HSP 84; HSP84; TSTA
• UniProt Entry Name: HS90B_MOUSE

Uniprot Description

HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Chaperone

Cellular Component: signalosome; cell surface; membrane; mitochondrion; basolateral plasma membrane; brush border membrane; cytoplasm; apical plasma membrane; intracellular; inclusion body; cytosol

Molecular Function: protein binding; GTP binding; unfolded protein binding; double-stranded RNA binding; nucleotide binding; dATP binding; sulfonylurea receptor binding; protein kinase binding; kinase binding; ATP binding

Biological Process: positive regulation of cell size; protein folding; positive regulation of protein import into nucleus, translocation; positive regulation of protein binding; response to stress; negative regulation of neuron apoptosis; placenta development