HIPK2 sirna

HIPK2 siRNA (Mouse)

Cat. Num. AAA8212439

• Gene Names: Hipk2; Stank; 1110014O20Rik; B230339E18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HIPK2; HIPK2 siRNA (Mouse); NBAK1; STANK; Homeodomain-interacting protein kinase 2; Nuclear body-associated kinase 1; Sialophorin tail-associated nuclear serine/threonine-protein kinase; HIPK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HIPK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1161

• Applicable Applications for HIPK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HIPK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HIPK2 sirna

siRNA to inhibit HIPK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209862937
• NCBI GeneID: 15258
• NCBI Accession #: NP_001129537.1
• NCBI GenBank Nucleotide #: NM_001136065.2
• UniProt Accession #: Q9QZR5; O88905; Q99P45; Q99P46; Q9D2E6; Q9D474; Q9EQL2; Q9QZR4
• Molecular Weight: 14,279 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 2
• NCBI Official Symbol: Hipk2
• NCBI Official Synonym Symbols: Stank; 1110014O20Rik; B230339E18Rik
• NCBI Protein Information: homeodomain-interacting protein kinase 2
• UniProt Protein Name: Homeodomain-interacting protein kinase 2
• Protein Family: Homeodomain-interacting protein kinase
• UniProt Gene Name: Hipk2
• UniProt Synonym Gene Names: Nbak1; Stank
• UniProt Entry Name: HIPK2_MOUSE

Uniprot Description

HIPK2: homeodomain interacting protein kinase 2 is a CMGC kinase of the DYRK family. Interacts with TRADD. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Inhibits cell growth and promotes apoptosis. Involved in transcriptional activation of TP53 and TP73. In response to TGFB, cooperates with DAXX to activate JNK. Phosphorylates the antiapoptotic factor CTBP1 and promotes its proteasomal degradation. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between TAK1 and NLK to promote the proteasomal degradation of MYB. Highly expressed in neuronal tissues, heart and kidney. Weakly expressed ubiquitously. Possible tumor suppressor. Activates p53 in response to UV light, causing growth arrest and apoptosis. Required for cisplatin-induced apoptosis. Downregulated in breast and thyroid carcinomas. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; CMGC group; DYRK family; HIPK subfamily

Cellular Component: nuclear body; PML body; cytoplasm; intracellular; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; DNA binding; transferase activity, transferring phosphorus-containing groups; virion binding; nucleotide binding; kinase activity; SMAD binding; transcription corepressor activity; ATP binding; protein kinase activity

Biological Process: voluntary musculoskeletal movement; positive regulation of protein binding; adult locomotory behavior; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; adult walking behavior; negative regulation of BMP signaling pathway; neuron differentiation; anterior/posterior pattern formation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; negative regulation of neuron apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of DNA binding; smoothened signaling pathway; transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; peptidyl-serine phosphorylation; embryonic retina morphogenesis in camera-type eye; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; embryonic camera-type eye morphogenesis; positive regulation of protein amino acid phosphorylation; phosphorylation; response to DNA damage stimulus