HEYL sirna

HEYL siRNA (Mouse)

Cat. Num. AAA8208751

• Gene Names: Heyl; Hey3; Hrt3; hesr3; bHLHb33
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HEYL; HEYL siRNA (Mouse); HESR3; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Hairy and enhancer of split-related protein 3; Hairy-related transcription factor 3; HRT-3; mHRT3; HEYL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HEYL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 326

• Applicable Applications for HEYL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HEYL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HEYL sirna

siRNA to inhibit HEYL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31560486
• NCBI GeneID: 56198
• NCBI Accession #: NP_038933.2
• NCBI GenBank Nucleotide #: NM_013905.3
• UniProt Accession #: Q9DBX7; Q9JJV6; Q9QXW8
• Molecular Weight: 34,929 Da
• NCBI Official Full Name: hairy/enhancer-of-split related with YRPW motif-like protein
• NCBI Official Synonym Full Names: hairy/enhancer-of-split related with YRPW motif-like
• NCBI Official Symbol: Heyl
• NCBI Official Synonym Symbols: Hey3; Hrt3; hesr3; bHLHb33
• NCBI Protein Information: hairy/enhancer-of-split related with YRPW motif-like protein
• UniProt Protein Name: Hairy/enhancer-of-split related with YRPW motif-like protein
• Protein Family: Hairy/enhancer-of-split related with YRPW motif-like protein
• UniProt Gene Name: Heyl
• UniProt Synonym Gene Names: Hesr3; Hrt3; HRT-3; mHRT3
• UniProt Entry Name: HEYL_MOUSE

Uniprot Description

HEYL: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family.

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; AF-1 domain binding; transcription corepressor activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; Notch signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation; peripheral nervous system development; mesenchymal cell development