HAX1 sirna

HAX1 siRNA (Mouse)

Cat. Num. AAA8220685

• Gene Names: Hax1; HAX-1; Hs1bp1; SIG-111; Silg111; mHAX-1s; HSP1BP-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HAX1; HAX1 siRNA (Mouse); HS1BP1; HCLS1-associated protein X-1; HS1-associating protein X-1; HAX-1; HS1-binding protein 1; HSP1BP-1; HAX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HAX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 280

• Applicable Applications for HAX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HAX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HAX1 sirna

siRNA to inhibit HAX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754160
• NCBI GeneID: 23897
• NCBI Accession #: NP_035956.1
• NCBI GenBank Nucleotide #: NM_011826.4
• UniProt Accession #: O35387; Q542F8
• Molecular Weight: 31,654 Da
• NCBI Official Full Name: HCLS1-associated protein X-1 isoform 1
• NCBI Official Synonym Full Names: HCLS1 associated X-1
• NCBI Official Symbol: Hax1
• NCBI Official Synonym Symbols: HAX-1; Hs1bp1; SIG-111; Silg111; mHAX-1s; HSP1BP-1
• NCBI Protein Information: HCLS1-associated protein X-1
• UniProt Protein Name: HCLS1-associated protein X-1
• Protein Family: HCLS1-associated protein
• UniProt Gene Name: Hax1
• UniProt Synonym Gene Names: Hs1bp1; HAX-1; HSP1BP-1
• UniProt Entry Name: HAX1_MOUSE

Uniprot Description

HAX1: Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools. Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3); also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. Belongs to the HAX1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Vesicle; Apoptosis; Mitochondrial

Cellular Component: transcription factor complex; sarcoplasmic reticulum; intracellular membrane-bound organelle; clathrin-coated vesicle; membrane; mitochondrion; lamellipodium; endoplasmic reticulum; cytoplasm; apical plasma membrane; cytoplasmic vesicle; nucleus; actin cytoskeleton

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding; interleukin-1 binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; regulation of actin filament polymerization; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of granulocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation