H2-AB1 sirna

H2-AB1 siRNA (Mouse)

Cat. Num. AAA8224087

• Gene Names: H2-Ab1; IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; AI845868
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: H2-AB1; H2-AB1 siRNA (Mouse); H2-IABETA; H-2 class II histocompatibility antigen A beta chain; H2-AB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: H2-AB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 265

• Applicable Applications for H2-AB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse H2-AB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for H2-AB1 sirna

siRNA to inhibit H2-AB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 294997230
• NCBI GeneID: 14961
• NCBI Accession #: NP_996988.2
• NCBI GenBank Nucleotide #: NM_207105.3
• UniProt Accession #: P14483; P01921; P06342; P06343; P06344; P06345; P06346
• Molecular Weight: 30,128 Da
• NCBI Official Full Name: histocompatibility 2, class II antigen A, beta 1
• NCBI Official Synonym Full Names: histocompatibility 2, class II antigen A, beta 1
• NCBI Official Symbol: H2-Ab1
• NCBI Official Synonym Symbols: IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; AI845868
• NCBI Protein Information: histocompatibility 2, class II antigen A, beta 1
• UniProt Protein Name: H-2 class II histocompatibility antigen, A beta chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: H2-Ab1
• UniProt Synonym Gene Names: H2-iabeta
• UniProt Entry Name: HB2A_MOUSE

Uniprot Description

HLA-DQB1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Cellular Component: Golgi apparatus; multivesicular body; cell surface; membrane; early endosome; integral to membrane; plasma membrane; intracellular; MHC class II protein complex; external side of plasma membrane

Molecular Function: toxin binding; protein binding; peptide antigen binding; protein heterodimerization activity; ubiquitin protein ligase binding; transcription factor binding

Biological Process: antigen processing and presentation; humoral immune response mediated by circulating immunoglobulin; antigen processing and presentation of peptide antigen; positive regulation of alpha-beta T cell activation; negative regulation of T cell proliferation; positive regulation of T-helper 1 type immune response; immune system process; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; immunoglobulin production during immune response; antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; positive regulation of antigen processing and presentation; B cell affinity maturation