GSTZ1 sirna

GSTZ1 siRNA (Mouse)

Cat. Num. AAA8215123

• Gene Names: Gstz1; MAAI
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GSTZ1; GSTZ1 siRNA (Mouse); MAAI; Maleylacetoacetate isomerase; GSTZ1-1; Glutathione S-transferase zeta 1; GSTZ1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GSTZ1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 161

• Applicable Applications for GSTZ1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GSTZ1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GSTZ1 sirna

siRNA to inhibit GSTZ1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 357527382
• NCBI GeneID: 14874
• NCBI Accession #: NP_001239484.1
• NCBI GenBank Nucleotide #: NM_001252555.1
• UniProt Accession #: Q9WVL0
• Molecular Weight: 24,275 Da
• NCBI Official Full Name: maleylacetoacetate isomerase isoform 2
• NCBI Official Synonym Full Names: glutathione transferase zeta 1 (maleylacetoacetate isomerase)
• NCBI Official Symbol: Gstz1
• NCBI Official Synonym Symbols: MAAI
• NCBI Protein Information: maleylacetoacetate isomerase
• UniProt Protein Name: Maleylacetoacetate isomerase
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: Gstz1
• UniProt Synonym Gene Names: Maai; MAAI
• UniProt Entry Name: MAAI_MOUSE

Uniprot Description

GSTZ1: Bifunctional enzyme showing minimal glutathione- conjugating activity with ethacrynic acid and 7-chloro-4- nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T- butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. Belongs to the GST superfamily. Zeta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; EC 2.5.1.18; Amino Acid Metabolism - tyrosine; Isomerase; Xenobiotic Metabolism - metabolism by cytochrome P450; Mitochondrial; EC 5.2.1.2; Transferase; Other Amino Acids Metabolism - glutathione

Cellular Component: mitochondrion; cytoplasm

Molecular Function: transferase activity; protein homodimerization activity; glutathione transferase activity; maleylacetoacetate isomerase activity; isomerase activity; catalytic activity

Biological Process: L-phenylalanine catabolic process; glutathione metabolic process; tyrosine catabolic process; metabolic process; aromatic amino acid family metabolic process