GPSM1 sirna

GPSM1 siRNA (Mouse)

Cat. Num. AAA8202966

• Gene Names: Gpsm1; Ags3; AW107933; 1810037C22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GPSM1; GPSM1 siRNA (Mouse); AGS3; G-protein-signaling modulator 1; Activator of G-protein signaling 3; GPSM1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GPSM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 613

• Applicable Applications for GPSM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GPSM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GPSM1 sirna

siRNA to inhibit GPSM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 312433991
• NCBI GeneID: 67839
• NCBI Accession #: NP_001186075.1
• NCBI GenBank Nucleotide #: NM_001199146.1
• UniProt Accession #: Q6IR34; Q61366; Q8BUK4; Q8BX78; Q8R0R9; A2AIX7; A2AIX8; A2AIX9
• Molecular Weight: 78,114 Da
• NCBI Official Full Name: G-protein-signaling modulator 1 isoform 2
• NCBI Official Synonym Full Names: G-protein signalling modulator 1 (AGS3-like, C. elegans)
• NCBI Official Symbol: Gpsm1
• NCBI Official Synonym Symbols: Ags3; AW107933; 1810037C22Rik
• NCBI Protein Information: G-protein-signaling modulator 1
• UniProt Protein Name: G-protein-signaling modulator 1
• Protein Family: G-protein-signaling modulator
• UniProt Gene Name: Gpsm1
• UniProt Synonym Gene Names: Ags3
• UniProt Entry Name: GPSM1_MOUSE

Uniprot Description

GPSM1: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G- protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction. Belongs to the GPSM family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: nucleoplasm; Golgi apparatus; membrane; endoplasmic reticulum; cytoplasm; plasma membrane

Molecular Function: protein binding; GDP-dissociation inhibitor activity; G-protein alpha-subunit binding; GTPase regulator activity

Biological Process: nervous system development; multicellular organismal development; cell differentiation; regulation of G-protein coupled receptor protein signaling pathway