GLMN sirna

GLMN siRNA (Mouse)

Cat. Num. AAA8223731

• Gene Names: Glmn; Fap48; Fap68; AW227515; 9330160J16Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLMN; GLMN siRNA (Mouse); FAP48; Glomulin; FK506-binding protein-associated protein; FAP; FKBP-associated protein; GLMN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GLMN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 596

• Applicable Applications for GLMN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GLMN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLMN sirna

siRNA to inhibit GLMN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 239985599
• NCBI GeneID: 170823
• NCBI Accession #: NP_001155210.1
• NCBI GenBank Nucleotide #: NM_001161738.1
• UniProt Accession #: Q8BZM1; Q3TFR5; Q99LB8
• Molecular Weight: 67,756 Da
• NCBI Official Full Name: glomulin isoform a
• NCBI Official Synonym Full Names: glomulin, FKBP associated protein
• NCBI Official Symbol: Glmn
• NCBI Official Synonym Symbols: Fap48; Fap68; AW227515; 9330160J16Rik
• NCBI Protein Information: glomulin
• UniProt Protein Name: Glomulin
• Protein Family: Glomulin
• UniProt Gene Name: Glmn
• UniProt Synonym Gene Names: Fap48; FAP
• UniProt Entry Name: GLMN_MOUSE

Uniprot Description

GLMN: Essential for normal development of the vasculature. May represent a naturally occurring ligand of the immunophilins FKBP59 and FKBP12. May function as an membrane anchoring protein. Isoform 1 may stimulate the p70S6K pathway. Isoform 2 may inhibit cell proliferation and increase IL2 production. Defects in GLMN are the cause of glomuvenous malformations (GVMs). GVMs are characterized by the presence of smooth-muscle-like glomus cells in the media surrounding distended vascular lumens. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: Cul2-RING ubiquitin ligase complex; cullin-RING ubiquitin ligase complex

Molecular Function: protein binding; hepatocyte growth factor receptor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase inhibitor activity; receptor binding

Biological Process: positive regulation of interleukin-2 biosynthetic process; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of gene expression, epigenetic; negative regulation of cell proliferation; muscle cell differentiation; cell surface receptor linked signal transduction; negative regulation of T cell proliferation; negative regulation of catalytic activity; neural tube closure; negative regulation of protein ubiquitination; vasculogenesis; positive regulation of phosphorylation; positive regulation of cytokine secretion