GLI2 sirna

GLI2 siRNA (Mouse)

Cat. Num. AAA8209040

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GLI2; GLI2 siRNA (Mouse); THP; Zinc finger protein GLI2; Tax helper protein; GLI2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GLI2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1544

• Applicable Applications for GLI2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GLI2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GLI2 sirna

siRNA to inhibit GLI2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487481
• NCBI GeneID: 14633
• NCBI Accession #: NP_001074594.1
• NCBI GenBank Nucleotide #: NM_001081125.1
• UniProt Accession #: Q0VGT2; E9PYJ4
• Molecular Weight: 165,031 Da
• NCBI Official Full Name: zinc finger protein GLI2
• NCBI Official Synonym Full Names: GLI-Kruppel family member GLI2
• NCBI Official Symbol: Gli2
• NCBI Protein Information: zinc finger protein GLI2
• UniProt Protein Name: Zinc finger protein GLI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: Gli2
• UniProt Entry Name: GLI2_MOUSE

Uniprot Description

GLI2: Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal. Interaction with ZIC. Belongs to the GLI C2H2-type zinc-finger protein family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; C2H2-type zinc finger protein; Transcription factor

Cellular Component: membrane; cytoplasm; motile primary cilium; nuclear speck; nucleus; cilium

Molecular Function: protein binding; DNA binding; nucleic acid binding; zinc ion binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; developmental growth; anatomical structure development; heart development; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; anatomical structure formation; chondrocyte differentiation; kidney development; embryonic gut development; hindbrain development; transcription, DNA-dependent; notochord regression; osteoblast development; branching morphogenesis of a tube; cerebellar cortex morphogenesis; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; tube development; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification; transcription from RNA polymerase II promoter; axon guidance; ventral midline development; spinal cord ventral commissure morphogenesis; spinal cord dorsal/ventral patterning; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; mammary gland development; positive regulation of cell proliferation; morphogenesis of an epithelium; cell differentiation; skeletal development; smoothened signaling pathway involved in ventral spinal cord interneuron specification; dorsoventral neural tube patterning; proximal/distal pattern formation; smoothened signaling pathway; floor plate formation; in utero embryonic development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; ventral spinal cord development; spinal cord motor neuron differentiation; pattern specification process; odontogenesis of dentine-containing teeth; osteoblast differentiation; cell proliferation; dorsal/ventral pattern formation; pituitary gland development; epidermal cell differentiation; hindgut morphogenesis; positive regulation of neuron differentiation; positive regulation of DNA replication; lung development; regulation of smoothened signaling pathway