GHRH sirna

GHRH siRNA (Mouse)

Cat. Num. AAA8226112

• Gene Names: Ghrh; Ghrf
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GHRH; GHRH siRNA (Mouse); Somatoliberin; Growth hormone-releasing factor; GRF; Growth hormone-releasing hormone; GHRH sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GHRH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 103

• Applicable Applications for GHRH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GHRH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GHRH sirna

siRNA to inhibit GHRH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6753984
• NCBI GeneID: 14601
• NCBI Accession #: NP_034415.1
• NCBI GenBank Nucleotide #: NM_010285.2
• UniProt Accession #: P16043
• Molecular Weight: 12,064 Da
• NCBI Official Full Name: somatoliberin
• NCBI Official Synonym Full Names: growth hormone releasing hormone
• NCBI Official Symbol: Ghrh
• NCBI Official Synonym Symbols: Ghrf
• NCBI Protein Information: somatoliberin
• UniProt Protein Name: Somatoliberin
• Protein Family: Somatoliberin
• UniProt Gene Name: Ghrh
• UniProt Synonym Gene Names: GRF; GHRH
• UniProt Entry Name: SLIB_MOUSE

Uniprot Description

GHRH: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; neuron projection; cell; extracellular region; terminal button; perikaryon; nerve terminal; intracellular; cytosol

Molecular Function: growth hormone-releasing hormone activity; protein binding; hormone activity; growth hormone-releasing hormone receptor binding

Biological Process: response to food; positive regulation of transcription, DNA-dependent; positive regulation of hormone secretion; positive regulation of cAMP metabolic process; positive regulation of multicellular organism growth; G-protein signaling, adenylate cyclase activating pathway; positive regulation of growth hormone secretion; G-protein signaling, coupled to cAMP nucleotide second messenger; growth hormone secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; circadian sleep/wake cycle, non-REM sleep; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; adenohypophysis development; positive regulation of circadian sleep/wake cycle, non-REM sleep