GDF5 sirna

GDF5 siRNA (Mouse)

Cat. Num. AAA8221532

• Gene Names: Gdf5; bp; brp; BMP-14; Cdmp-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GDF5; GDF5 siRNA (Mouse); BMP14; BP; GDF-5; Growth/differentiation factor 5; Bone morphogenetic protein 14; BMP-14; GDF5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GDF5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 495

• Applicable Applications for GDF5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GDF5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GDF5 sirna

siRNA to inhibit GDF5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224922797
• NCBI GeneID: 14563
• NCBI Accession #: NP_032135.2
• NCBI GenBank Nucleotide #: NM_008109.2
• UniProt Accession #: P43027; A2ARK2
• Molecular Weight: 54,895 Da
• NCBI Official Full Name: growth/differentiation factor 5
• NCBI Official Synonym Full Names: growth differentiation factor 5
• NCBI Official Symbol: Gdf5
• NCBI Official Synonym Symbols: bp; brp; BMP-14; Cdmp-1
• NCBI Protein Information: growth/differentiation factor 5
• UniProt Protein Name: Growth/differentiation factor 5
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: Gdf5
• UniProt Synonym Gene Names: Bmp14; Bp; Gdf-5; GDF-5; BMP-14
• UniProt Entry Name: GDF5_MOUSE

Uniprot Description

GDF5: a cytokine that is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. These cytokines are characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Binds to bone morphogenetic protein receptors (BMPRs), a family of transmembrane serine/threonine kinases. BMPRs are involved in bone and cartilage formation. Chondrogenic signaling is mediated by the high-affinity receptor BMPR1B. Defects in GDF5 are the cause of acromesomelic chondrodysplasia Grebe type (AMDG), acromesomelic chondrodysplasia Hunter-Thompson type (AMDH), brachydactyly type C (BDC), Du Pan syndrome (DPS), symphalangism proximal syndrome (SYM1), multiple synostoses syndrome type 2 (SYNS2), and brachydactyly type A2 (BDA2). Genetic variations in GDF5 are associated with susceptibility to osteoarthritis type 5 (OS5). Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; membrane; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: hindlimb morphogenesis; regulation of multicellular organism growth; BMP signaling pathway; forelimb morphogenesis; positive regulation of chondrocyte differentiation; regulation of MAPKKK cascade; cartilage development; transmembrane receptor protein serine/threonine kinase signaling pathway; chondrocyte differentiation; negative regulation of neuron apoptosis; positive regulation of neuron differentiation; cell development; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; growth