GCAT sirna
GCAT siRNA (Mouse)
Gene Names
Gcat; Kbl; AI526977
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
GCAT; GCAT siRNA (Mouse); KBL; 2-amino-3-ketobutyrate coenzyme A ligase mitochondrial; AKB ligase; Aminoacetone synthase; Glycine acetyltransferase; GCAT sirna
Host
Synthetic
Reactivity
Mouse
Specificity
GCAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
416
Applicable Applications for GCAT sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GCAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for GCAT sirna
siRNA to inhibit GCAT expression using RNA interference
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,931 Da
NCBI Official Full Name
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial isoform a
NCBI Official Synonym Full Names
glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase)
NCBI Official Symbol
Gcat
NCBI Official Synonym Symbols
Kbl; AI526977
NCBI Protein Information
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Protein Name
2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
UniProt Gene Name
Gcat
UniProt Synonym Gene Names
Kbl; AKB ligase
UniProt Entry Name
KBL_MOUSE
Uniprot Description
GCAT: The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. This gene encodes the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The encoded enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 14. [provided by RefSeq, Jan 2010]
Protein type: Amino Acid Metabolism - glycine, serine and threonine; Transferase; EC 2.3.1.29; Mitochondrial
Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane
Research Articles on GCAT
Similar Products
Product Notes
The GCAT gcat (Catalog #AAA8237965) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The GCAT siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GCAT can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the GCAT gcat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GCAT, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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