GAPVD1 sirna

GAPVD1 siRNA (Mouse)

Cat. Num. AAA8208917

• Gene Names: Gapvd1; RAP6; RME-6; Gapex-5; AW108497; mKIAA1521; 2010005B09Rik; 4432404J10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GAPVD1; GAPVD1 siRNA (Mouse); GAPEX5; KIAA1521; GTPase-activating protein and VPS9 domain-containing protein 1; GAPex-5; Rab5-activating protein 6; GAPVD1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GAPVD1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1437

• Applicable Applications for GAPVD1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GAPVD1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GAPVD1 sirna

siRNA to inhibit GAPVD1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40254514
• NCBI GeneID: 66691
• NCBI Accession #: NP_079985.2
• NCBI GenBank Nucleotide #: NM_025709.2
• UniProt Accession #: Q6PAR5; Q3TNS1; Q3UDL0; Q3UYD5; Q6ZPP0; Q80V37; Q80ZK4; A0PJI8; A2AR09; A2AR10; A2AR17; A2AR18
• Molecular Weight: 162,273 Da
• NCBI Official Full Name: GTPase-activating protein and VPS9 domain-containing protein 1
• NCBI Official Synonym Full Names: GTPase activating protein and VPS9 domains 1
• NCBI Official Symbol: Gapvd1
• NCBI Official Synonym Symbols: RAP6; RME-6; Gapex-5; AW108497; mKIAA1521; 2010005B09Rik; 4432404J10Rik
• NCBI Protein Information: GTPase-activating protein and VPS9 domain-containing protein 1
• UniProt Protein Name: GTPase-activating protein and VPS9 domain-containing protein 1
• Protein Family: GTPase-activating protein and VPS9 domain-containing protein
• UniProt Gene Name: Gapvd1
• UniProt Synonym Gene Names: Gapex5; Kiaa1521
• UniProt Entry Name: GAPD1_MOUSE

Uniprot Description

GAPVD1: an endosomal G protein regulator that acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF). Has GEF activity for Rab5 and GAP activity for Ras. Participates in various processes such as endocytosis, insulin receptor internalization and GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between GLUT4 storage vesicles and early endosomes, retaining GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing GLUT4 from intracellular storage vesicles. Recruited to the plasma membrane by TRIP10 in response to insulin. Involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Recruited to the plasma membrane by TRIP10 in response to insulin.

Protein type: GEFs, Rab; GAPs, Ras; GEFs; Vesicle; GAPs

Cellular Component: membrane; intracellular; cytosol; endosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; GTPase activating protein binding; GTPase activator activity

Biological Process: regulation of protein transport; regulation of GTPase activity; endocytosis; signal transduction; positive regulation of GTPase activity