GALK1 sirna

GALK1 siRNA (Mouse)

Cat. Num. AAA8212880

• Gene Names: Galk1; Gk; Glk; GALK; Glk1; AA409894
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GALK1; GALK1 siRNA (Mouse); GALK; GLK; Galactokinase; Galactose kinase; GALK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: GALK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 392

• Applicable Applications for GALK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GALK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for GALK1 sirna

siRNA to inhibit GALK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 93102413
• NCBI GeneID: 14635
• NCBI Accession #: NP_058601.2
• NCBI GenBank Nucleotide #: NM_016905.2
• UniProt Accession #: Q9R0N0; Q9JIA6
• Molecular Weight: 42,295 Da
• NCBI Official Full Name: galactokinase
• NCBI Official Synonym Full Names: galactokinase 1
• NCBI Official Symbol: Galk1
• NCBI Official Synonym Symbols: Gk; Glk; GALK; Glk1; AA409894
• NCBI Protein Information: galactokinase
• UniProt Protein Name: Galactokinase
• UniProt Gene Name: Galk1
• UniProt Synonym Gene Names: Galk; Glk
• UniProt Entry Name: GALK1_MOUSE

Uniprot Description

GALK1: Major enzyme for galactose metabolism. Defects in GALK1 are the cause of galactosemia II (GALCT2). Galactosemia II is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses. Belongs to the GHMP kinase family. GalK subfamily.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Kinase, other; EC 2.7.1.6; Carbohydrate Metabolism - galactose

Cellular Component: cytoplasm

Molecular Function: transferase activity; galactokinase activity; protein binding; galactose binding; nucleotide binding; kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding

Biological Process: metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; galactitol metabolic process; galactose metabolic process; phosphorylation