FZD6 sirna

FZD6 siRNA (Mouse)

Cat. Num. AAA8203497

• Gene Names: Fzd6; Fz6
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FZD6; FZD6 siRNA (Mouse); Frizzled-6; Fz-6; mFz6; FZD6 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FZD6 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 709

• Applicable Applications for FZD6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FZD6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FZD6 sirna

siRNA to inhibit FZD6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 242332484
• NCBI GeneID: 14368
• NCBI Accession #: NP_001155966.1
• NCBI GenBank Nucleotide #: NM_001162494.1
• UniProt Accession #: Q61089
• Molecular Weight: 79,082 Da
• NCBI Official Full Name: frizzled-6
• NCBI Official Synonym Full Names: frizzled homolog 6 (Drosophila)
• NCBI Official Symbol: Fzd6
• NCBI Official Synonym Symbols: Fz6
• NCBI Protein Information: frizzled-6
• UniProt Protein Name: Frizzled-6
• Protein Family: Frizzled
• UniProt Gene Name: Fzd6
• UniProt Synonym Gene Names: Fz-6; mFz6
• UniProt Entry Name: FZD6_MOUSE

Uniprot Description

FZD6: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Detected in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, thymus, prostate, testis, ovary, small intestine and colon. In the fetus, expressed in brain, lung, liver and kidney. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, Fz/Smo family

Cellular Component: membrane; apicolateral plasma membrane; apical part of cell; plasma membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; protein binding; Wnt receptor activity; signal transducer activity; transmembrane receptor activity

Biological Process: platelet activation; nervous system development; inner ear morphogenesis; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; multicellular organismal development; Wnt receptor signaling pathway through beta-catenin; signal transduction; G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; hair follicle development; midbrain development; neural tube closure; establishment of planar polarity; cell proliferation in midbrain