FUT2 sirna

FUT2 siRNA (Mouse)

Cat. Num. AAA8208986

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FUT2; FUT2 siRNA (Mouse); SEC2; Galactoside 2-alpha-L-fucosyltransferase 2; Alpha(1.2)FT 2; Fucosyltransferase 2; GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 2; Secretory blood group protein 2; FUT2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FUT2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 347

• Applicable Applications for FUT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FUT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FUT2 sirna

siRNA to inhibit FUT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 431822426
• NCBI GeneID: 14344
• NCBI Accession #: NP_001258922.1
• NCBI GenBank Nucleotide #: NM_001271993.1
• UniProt Accession #: Q9JL27
• Molecular Weight: 39,243 Da
• NCBI Official Full Name: galactoside 2-alpha-L-fucosyltransferase 2
• NCBI Official Synonym Full Names: fucosyltransferase 2
• NCBI Official Symbol: Fut2
• NCBI Protein Information: galactoside 2-alpha-L-fucosyltransferase 2
• UniProt Protein Name: Galactoside 2-alpha-L-fucosyltransferase 2
• Protein Family: Fucosyltransferase
• UniProt Gene Name: Fut2
• UniProt Synonym Gene Names: Sec2
• UniProt Entry Name: FUT2_MOUSE

NCBI Description

This gene is one of three genes in mouse which encode a galactoside 2-L-fucosyltransferase. These genes differ in their developmental- and tissue-specific expression. The encoded type II membrane protein is anchored in the Golgi apparatus and controls the final step in the creation of alpha (1,2) fucosylated carbhohydrates by the addition of a terminal fucose in an alpha (1,2) linkage. This enzyme is involved in the synthesis of the Lewis antigen as well as the H-antigen, a precursor of the A and B antigens of the ABH histo-blood group. The biological function of the fucosylated carbhohydrate products is thought to involve cell-adhesion and interactions with microorganisms. Disruption of this gene results in altered glycosylation of gastric mucosa and uterine epithelia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

FUT2: Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble A and B antigen synthesis pathway. H and Se enzymes fucosylate the same acceptor substrates but exhibit different Km values. Belongs to the glycosyltransferase 11 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - globo series; Transferase; EC 2.4.1.69; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; galactoside 2-alpha-L-fucosyltransferase activity; fucosyltransferase activity

Biological Process: carbohydrate metabolic process; protein amino acid glycosylation; L-fucose catabolic process