FBXW7 sirna

FBXW7 siRNA (Mouse)

Cat. Num. AAA8234176

• Gene Names: Fbxw7; AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxw6; Fbxo30; SEL-10; 1110001A17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FBXW7; FBXW7 siRNA (Mouse); FBW7; FBWD6; FBXW6; F-box/WD repeat-containing protein 7; F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein Fbxw6; F-box-WD40 repeat protein 6; SEL-10; FBXW7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FBXW7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 629

• Applicable Applications for FBXW7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FBXW7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FBXW7 sirna

siRNA to inhibit FBXW7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21218434
• NCBI GeneID: 50754
• NCBI Accession #: NP_536353.2
• NCBI GenBank Nucleotide #: NM_080428.3
• UniProt Accession #: Q8VBV4; Q8CCS5; Q8VHP4; A2RRI5
• Molecular Weight: 70,547 Da
• NCBI Official Full Name: F-box/WD repeat-containing protein 7 isoform 2
• NCBI Official Synonym Full Names: F-box and WD-40 domain protein 7
• NCBI Official Symbol: Fbxw7
• NCBI Official Synonym Symbols: AGO; Cdc4; Fbw7; Fbwd6; Fbx30; Fbxw6; Fbxo30; SEL-10; 1110001A17Rik
• NCBI Protein Information: F-box/WD repeat-containing protein 7
• UniProt Protein Name: F-box/WD repeat-containing protein 7
• Protein Family: F-box/WD repeat-containing protein
• UniProt Gene Name: Fbxw7
• UniProt Entry Name: FBXW7_MOUSE

Uniprot Description

FBXW7 iso3: a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins including MYC, cyclin E, NOTCH1-released notch intracellular domain (NICD), JUN, and probably PSEN1. Recognizes and binds to phosphorylated target proteins. Other components of the SCF(FBXW7) complex are sCUL1, RBX1, and SKP1A. Defects in FBXW7 may be a cause of breast cancer. The homolog of Cdc4, one of the yeast genes that regulate sister chromatid cohesion. Down-regulation or disruption of FBXW7 resulted in abnormal sister chromatid cohesion in human cells. Four alternatively spliced isoforms have been described.

Protein type: Nucleolus; Ubiquitin conjugating system; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; Golgi apparatus; protein complex; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; nucleolus; integral to membrane; SCF ubiquitin ligase complex; nucleus

Molecular Function: protein binding, bridging; identical protein binding; cyclin binding; protein binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of epidermal growth factor receptor activity; regulation of transcription, DNA-dependent; protein stabilization; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; sister chromatid cohesion; negative regulation of DNA endoreduplication; protein ubiquitination; vasculogenesis; response to DNA damage stimulus; lung development