FBLN1 sirna

FBLN1 siRNA (Mouse)

Cat. Num. AAA8216501

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FBLN1; FBLN1 siRNA (Mouse); Fibulin-1; FIBL-1; Basement-membrane protein 90; BM-90; FBLN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FBLN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: –

• Applicable Applications for FBLN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FBLN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FBLN1 sirna

siRNA to inhibit FBLN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 168693628
• NCBI GeneID: 14114
• NCBI Accession #: NP_034310.2
• NCBI GenBank Nucleotide #: NM_010180.2
• UniProt Accession #: Q08879; Q08878; Q8C3B1; Q91ZC9; Q922K8
• Molecular Weight: – Da
• NCBI Official Full Name: fibulin-1
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: Fbln1
• NCBI Protein Information: fibulin-1
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: Fbln1
• UniProt Synonym Gene Names: FIBL-1; BM-90
• UniProt Entry Name: FBLN1_MOUSE

Uniprot Description

FBLN1: Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation. Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane

Molecular Function: protein binding; protease activator activity; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis