FANCL sirna

FANCL siRNA (Mouse)

Cat. Num. AAA8212435

• Gene Names: Fancl; Pog; gcd; Phf9; AW554273; 2010322C19Rik; B230118H11Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FANCL; FANCL siRNA (Mouse); PHF9; POG; E3 ubiquitin-protein ligase FANCL; Fanconi anemia group L protein homolog; Proliferation of germ cells protein; FANCL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: FANCL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for FANCL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FANCL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FANCL sirna

siRNA to inhibit FANCL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 472235270
• NCBI GeneID: 67030
• NCBI Accession #: NP_001264202.1
• NCBI GenBank Nucleotide #: NM_001277273.1
• UniProt Accession #: Q9CR14; Q3TGY2; Q9D017
• Molecular Weight: 41,902 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase FANCL isoform 2
• NCBI Official Synonym Full Names: Fanconi anemia, complementation group L
• NCBI Official Symbol: Fancl
• NCBI Official Synonym Symbols: Pog; gcd; Phf9; AW554273; 2010322C19Rik; B230118H11Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase FANCL
• UniProt Protein Name: E3 ubiquitin-protein ligase FANCL
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Fancl
• UniProt Synonym Gene Names: Phf9; Pog
• UniProt Entry Name: FANCL_MOUSE

NCBI Description

This gene encodes the complementation group L subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes. The FA complex is necessary for protection against DNA damage. This gene product, an E3 ubiquitin ligase, catalyzes and is required for the monoubiquitination of the protein encoded by the Fanconi anemia, complementation group D2 gene, a critical step in the FA pathway (PMID: 12973351, 21229326). In mouse, mutations of this E3 ubiquitin ligase gene can lead to infertility in adult males and females, and a deletion of this gene can cause embryonic lethality in some genetic backgrounds. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

FANCL: Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth. Defects in FANCL are the cause of Fanconi anemia complementation group L (FANCL). FANCL is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19

Cellular Component: cytoplasm; nuclear envelope; nucleus

Molecular Function: protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin protein ligase binding; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein monoubiquitination; gamete generation; protein ubiquitination; DNA repair; response to DNA damage stimulus; regulation of cell proliferation