ERO1LB sirna

ERO1LB siRNA (Mouse)

Cat. Num. AAA8218609

• Gene Names: Ero1lb; AI447560; ero1-beta; 1300013B24Rik; 1700065B09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERO1LB; ERO1LB siRNA (Mouse); ERO1-like protein beta; ERO1-L-beta; Endoplasmic reticulum oxidoreductin-1-like protein B; Oxidoreductin-1-L-beta; ERO1LB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERO1LB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 467

• Applicable Applications for ERO1LB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERO1LB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERO1LB sirna

siRNA to inhibit ERO1LB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 38348230
• NCBI GeneID: 67475
• NCBI Accession #: NP_080460.2
• NCBI GenBank Nucleotide #: NM_026184.2
• UniProt Accession #: Q8R2E9; Q14DN0
• Molecular Weight: 53,518 Da
• NCBI Official Full Name: ERO1-like protein beta
• NCBI Official Synonym Full Names: ERO1-like beta (S. cerevisiae)
• NCBI Official Symbol: Ero1lb
• NCBI Official Synonym Symbols: AI447560; ero1-beta; 1300013B24Rik; 1700065B09Rik
• NCBI Protein Information: ERO1-like protein beta
• UniProt Protein Name: ERO1-like protein beta
• UniProt Gene Name: Ero1lb
• UniProt Synonym Gene Names: ERO1-L-beta
• UniProt Entry Name: ERO1B_MOUSE

Uniprot Description

ERO1LB: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the being a source of oxidative stress. Required for the folding of cell, thereby being a source of oxidative stress. Belongs to the EROs family.

Protein type: Oxidoreductase; Endoplasmic reticulum; EC 1.8.4.-

Cellular Component: membrane; endoplasmic reticulum; cell

Molecular Function: peptide-methionine-(S)-S-oxide reductase activity; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; peptide-methionine (R)-S-oxide reductase activity; oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: insulin processing; extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; transport; cell redox homeostasis; glucose homeostasis