ERCC3 sirna

ERCC3 siRNA (Mouse)

Cat. Num. AAA8232485

• Gene Names: Ercc3; XPB; Ercc-3; BTF2 p89
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ERCC3; ERCC3 siRNA (Mouse); XPB; XPBC; TFIIH basal transcription factor complex helicase XPB subunit; Basic transcription factor 2 89 kDa subunit; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair protein complementing XP-B cells; TFIIH 89 kDa subunit; Xeroderma pigmentosum group B-complementing protein; ERCC3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ERCC3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 783

• Applicable Applications for ERCC3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ERCC3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ERCC3 sirna

siRNA to inhibit ERCC3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19526800
• NCBI GeneID: 13872
• NCBI Accession #: NP_598419.1
• NCBI GenBank Nucleotide #: NM_133658.1
• UniProt Accession #: P49135
• Molecular Weight: 89,126 Da
• NCBI Official Full Name: TFIIH basal transcription factor complex helicase XPB subunit
• NCBI Official Synonym Full Names: excision repair cross-complementing rodent repair deficiency, complementation group 3
• NCBI Official Symbol: Ercc3
• NCBI Official Synonym Symbols: XPB; Ercc-3; BTF2 p89
• NCBI Protein Information: TFIIH basal transcription factor complex helicase XPB subunit
• UniProt Protein Name: TFIIH basal transcription factor complex helicase XPB subunit
• Protein Family: Probable DNA repair helicase
• UniProt Gene Name: Ercc3
• UniProt Synonym Gene Names: Xpb; Xpbc; BTF2 p89
• UniProt Entry Name: ERCC3_MOUSE

Uniprot Description

XPB: ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2. Belongs to the helicase family. RAD25/XPB subfamily.

Protein type: DNA repair, damage; Helicase; Transcription factor; EC 3.6.4.12

Cellular Component: nucleoplasm; holo TFIIH complex; nucleus

Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; GTP binding; hydrolase activity; ATPase activity; nucleotide binding; 3'-5' DNA helicase activity; dATP binding; protein N-terminus binding; helicase activity; peptide binding; protein kinase activity; RNA polymerase subunit kinase activity; ATP-dependent DNA helicase activity; DNA binding; ATP binding

Biological Process: transcription from RNA polymerase II promoter; DNA topological change; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; nucleotide-excision repair, DNA incision; hair cell differentiation; DNA repair; nucleotide-excision repair, DNA duplex unwinding; protein amino acid phosphorylation; protein localization; regulation of transcription, DNA-dependent; UV protection; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; response to DNA damage stimulus; response to UV