EIF4A3 sirna

EIF4A3 siRNA (Mouse)

Cat. Num. AAA8226001

• Gene Names: Eif4a3; Ddx48; eIF4A-III; mKIAA0111; 2400003O03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF4A3; EIF4A3 siRNA (Mouse); DDX48; Eukaryotic initiation factor 4A-III; eIF-4A-III; eIF4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic translation initiation factor 4A isoform 3; EIF4A3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF4A3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 411

• Applicable Applications for EIF4A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF4A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF4A3 sirna

siRNA to inhibit EIF4A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20149756
• NCBI GeneID: 192170
• NCBI Accession #: NP_619610.1
• NCBI GenBank Nucleotide #: NM_138669.1
• UniProt Accession #: Q91VC3; Q3TEZ8; Q3UD29; Q8BVY3; B2RY38
• Molecular Weight: 46,840 Da
• NCBI Official Full Name: eukaryotic initiation factor 4A-III
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4A3
• NCBI Official Symbol: Eif4a3
• NCBI Official Synonym Symbols: Ddx48; eIF4A-III; mKIAA0111; 2400003O03Rik
• NCBI Protein Information: eukaryotic initiation factor 4A-III
• UniProt Protein Name: Eukaryotic initiation factor 4A-III
• Protein Family: Eukaryotic initiation factor
• UniProt Gene Name: Eif4a3
• UniProt Synonym Gene Names: Ddx48; eIF-4A-III; eIF4A-III
• UniProt Entry Name: IF4A3_MOUSE

Uniprot Description

DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: RNA splicing; RNA-binding; EC 3.6.4.13; Helicase; Spliceosome

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: mRNA binding; ATP-dependent RNA helicase activity; poly(A) binding; ATP binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; positive regulation of translation; negative regulation of translation; mRNA catabolic process, nonsense-mediated decay; positive regulation of transcription from RNA polymerase II promoter; embryonic cranial skeleton morphogenesis