EIF2B2 sirna

EIF2B2 siRNA (Mouse)

Cat. Num. AAA8216272

• Gene Names: Eif2b2; EIF2B; C85417; AA409345; EIF-2Bbeta
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: EIF2B2; EIF2B2 siRNA (Mouse); Translation initiation factor eIF-2B subunit beta; eIF-2B GDP-GTP exchange factor subunit beta; EIF2B2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: EIF2B2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 351

• Applicable Applications for EIF2B2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EIF2B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for EIF2B2 sirna

siRNA to inhibit EIF2B2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21703888
• NCBI GeneID: 217715
• NCBI Accession #: NP_663420.1
• NCBI GenBank Nucleotide #: NM_145445.3
• UniProt Accession #: Q99LD9
• Molecular Weight: 38,898 Da
• NCBI Official Full Name: translation initiation factor eIF-2B subunit beta
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B, subunit 2 beta
• NCBI Official Symbol: Eif2b2
• NCBI Official Synonym Symbols: EIF2B; C85417; AA409345; EIF-2Bbeta
• NCBI Protein Information: translation initiation factor eIF-2B subunit beta
• UniProt Protein Name: Translation initiation factor eIF-2B subunit beta
• Protein Family: Translation initiation factor
• UniProt Gene Name: Eif2b2
• UniProt Entry Name: EI2BB_MOUSE

NCBI Description

This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. Mutations in the human gene are associated with ovarioleukodystrophy and leukoencephalopathy with vanishing white matter. [provided by RefSeq, Sep 2015]

Uniprot Description

eIF2B-beta: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy.

Protein type: Translation; RNA-binding; GEFs, misc.; GEFs; Cell development/differentiation

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm

Molecular Function: GTP binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; ATP binding

Biological Process: myelination; response to peptide hormone stimulus; central nervous system development; translation; cellular response to stimulus; cellular metabolic process; ovarian follicle development; response to heat; translational initiation; response to glucose stimulus; oligodendrocyte development; positive regulation of GTPase activity; regulation of translational initiation