DUSP3 sirna

DUSP3 siRNA (Mouse)

Cat. Num. AAA8218152

• Gene Names: Dusp3; VHR; T-DSP11; 2210015O03Rik; 5031436O03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DUSP3; DUSP3 siRNA (Mouse); Dual specificity protein phosphatase 3; T-DSP11; Vaccinia H1-related phosphatase; VHR; DUSP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DUSP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 185

• Applicable Applications for DUSP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DUSP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DUSP3 sirna

siRNA to inhibit DUSP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21312314
• NCBI GeneID: 72349
• NCBI Accession #: NP_082483.1
• NCBI GenBank Nucleotide #: NM_028207.3
• UniProt Accession #: Q9D7X3
• Molecular Weight: 20,472 Da
• NCBI Official Full Name: dual specificity protein phosphatase 3
• NCBI Official Synonym Full Names: dual specificity phosphatase 3 (vaccinia virus phosphatase VH1-related)
• NCBI Official Symbol: Dusp3
• NCBI Official Synonym Symbols: VHR; T-DSP11; 2210015O03Rik; 5031436O03Rik
• NCBI Protein Information: dual specificity protein phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 3
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: Dusp3
• UniProt Synonym Gene Names: VHR
• UniProt Entry Name: DUS3_MOUSE

Uniprot Description

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.16; Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; cytoplasm; immunological synapse; cytosol; nucleus

Molecular Function: MAP kinase phosphatase activity; hydrolase activity; protein tyrosine/serine/threonine phosphatase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; protein kinase binding; phosphoprotein phosphatase activity

Biological Process: negative regulation of JNK cascade; dephosphorylation; negative regulation of MAPKKK cascade; in utero embryonic development; negative regulation of T cell receptor signaling pathway; positive regulation of mitotic cell cycle; protein amino acid dephosphorylation; negative regulation of T cell activation; inactivation of MAPK activity