DSTYK sirna

DSTYK siRNA (Mouse)

Cat. Num. AAA8234347

• Gene Names: Dstyk; Ripk5; C820013G01; A930019K20Rik; C430014H23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DSTYK; DSTYK siRNA (Mouse); RIPK5; Dual serine/threonine and tyrosine protein kinase; Dusty protein kinase; Dusty PK; Receptor-interacting serine/threonine-protein kinase 5; DSTYK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DSTYK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 927

• Applicable Applications for DSTYK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DSTYK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DSTYK sirna

siRNA to inhibit DSTYK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 40556610
• NCBI GeneID: 213452
• NCBI Accession #: NP_766104.2
• NCBI GenBank Nucleotide #: NM_172516.4
• UniProt Accession #: Q6XUX1; Q3ULK4; Q5EBN5; Q8C7D4; Q8C923; Q9CTP7
• Molecular Weight: 18,814 Da
• NCBI Official Full Name: dual serine/threonine and tyrosine protein kinase
• NCBI Official Synonym Full Names: dual serine/threonine and tyrosine protein kinase
• NCBI Official Symbol: Dstyk
• NCBI Official Synonym Symbols: Ripk5; C820013G01; A930019K20Rik; C430014H23Rik
• NCBI Protein Information: dual serine/threonine and tyrosine protein kinase
• UniProt Protein Name: Dual serine/threonine and tyrosine protein kinase
• Protein Family: Dual serine/threonine and tyrosine protein kinase
• UniProt Gene Name: Dstyk
• UniProt Synonym Gene Names: Ripk5; Dusty PK
• UniProt Entry Name: DUSTY_MOUSE

Uniprot Description

DSTYK: a serine/threonine protein kinase of the 'Other-Unique' family, most closely related to kinases of the TKL family including IRAK and RIPK. Overexpression induces apoptotic cell death. The caspase inhibitor crmA apparently blocks DNA fragmentation but not apoptosis, suggesting that SgK496 may induce both caspase-dependent apoptosis and caspase-independent cell death. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.12.1; Motility/polarity/chemotaxis; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; Other-Unique family

Cellular Component: membrane; basolateral plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; cell junction

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: positive regulation of kinase activity; positive regulation of fibroblast growth factor receptor signaling pathway; phosphorylation; protein amino acid phosphorylation