DOCK1 sirna

DOCK1 siRNA (Mouse)

Cat. Num. AAA8205802

• Gene Names: Dock1; AI854900; 9130006G06Rik; D630004B07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DOCK1; DOCK1 siRNA (Mouse); Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180; DOCK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DOCK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1865

• Applicable Applications for DOCK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DOCK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DOCK1 sirna

siRNA to inhibit DOCK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 88853584
• NCBI GeneID: 330662
• NCBI Accession #: NP_001028592.1
• NCBI GenBank Nucleotide #: NM_001033420.2
• UniProt Accession #: Q8BUR4; Q3URT8; Q3UY80; Q4QQL8; Q6PD14; Q8BVC9; Q91Z55; Q922V1
• Molecular Weight: 182,628 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 1
• NCBI Official Synonym Full Names: dedicator of cytokinesis 1
• NCBI Official Symbol: Dock1
• NCBI Official Synonym Symbols: AI854900; 9130006G06Rik; D630004B07Rik
• NCBI Protein Information: dedicator of cytokinesis protein 1
• UniProt Protein Name: Dedicator of cytokinesis protein 1
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: Dock1
• UniProt Synonym Gene Names: DOCK180
• UniProt Entry Name: DOCK1_MOUSE

Uniprot Description

DOCK1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1. Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon. Belongs to the DOCK family.

Protein type: GEFs; Motility/polarity/chemotaxis; Cytoskeletal; Adaptor/scaffold; GEFs, Rac/Rho

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; SH3 domain binding

Biological Process: cell migration; apoptosis; small GTPase mediated signal transduction; hemopoietic progenitor cell differentiation; phagocytosis