DHH sirna

DHH siRNA (Mouse)

Cat. Num. AAA8203569

• Gene Names: Dhh; C78960
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DHH; DHH siRNA (Mouse); Desert hedgehog protein; HHG-3; DHH sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DHH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 396

• Applicable Applications for DHH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DHH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DHH sirna

siRNA to inhibit DHH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6681181
• NCBI GeneID: 13363
• NCBI Accession #: NP_031883.1
• NCBI GenBank Nucleotide #: NM_007857.4
• UniProt Accession #: Q61488
• Molecular Weight: 43,542 Da
• NCBI Official Full Name: desert hedgehog protein
• NCBI Official Synonym Full Names: desert hedgehog
• NCBI Official Symbol: Dhh
• NCBI Official Synonym Symbols: C78960
• NCBI Protein Information: desert hedgehog protein
• UniProt Protein Name: Desert hedgehog protein
• Protein Family: Desert hedgehog protein
• UniProt Gene Name: Dhh
• UniProt Synonym Gene Names: DHH
• UniProt Entry Name: DHH_MOUSE

Uniprot Description

DHH: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD). PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM); also known as male- limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. Belongs to the hedgehog family.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Cellular Component: membrane; plasma membrane; extracellular region

Molecular Function: peptidase activity; protein binding; hydrolase activity; zinc ion binding; metal ion binding; patched binding; calcium ion binding

Biological Process: intein-mediated protein splicing; Leydig cell differentiation; cell-cell signaling; male sex determination; multicellular organismal development; regulation of steroid biosynthetic process; cell communication; proteolysis; spermatid development